Background number of DNA extraction by boiling

 

Background & aim of work:
Tuberculosis
(TB) is a critical contagious disease that primarily affects and represents a
significant public health problem all over
the world. The ultimate diagnosis of Mycobacterium tuberculosis (MTB) is mainly
by microbiological tests including microscopy, culture and molecular methods.
Both microscopy and culture have many drawbacks as low sensitivity for smear
and lateness for culture. The initial step in the fast identification of TB by
the molecular method is DNA extraction.

 

Methods:   In this study, direct DNA extraction methods
of MBT from sputum samples were done by solid, digestion and phenol methods, while DNA
extraction of  MBT from culture isolates
were done by solid, boiling and Cetyl trimethylammonium bromide ( CTAB)
methods.

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Results:  Among 32
positive sputum samples, the number of DNA extraction by phenol-chloroform-isoamyl alcohol method was
21/32 (65.62%), followed by digestion method 14/32(43.75%) and the least one
was solid method 1/32(2.5%). The
number of positive extracted DNA were significantly (P